We also used the measured 24,25[(OH).sub.2][D.sub.3] concentration in
sera from the NANS subset (with and without the mean factor of 2.79) to adjust the immunoassay-measured total serum 25(OH)D concentration and compare the mean to that from LC-MS/MS estimates.
In fact, there is no reliable method for accurately measuring the concentration of bilirubin in calibrators and controls consisting of UBIL, DTB, or both in bovine
sera from commercial sources.
We obtained convalescent
sera 19-21 days after the acute
sera; these samples were tested in parallel with the acute
sera.
(2004), the analyzed
sera were not collected in such a way as to be representative of the general U.S.
With the Vitros F[T.sub.3] assay, F[T.sub.3] concentrations in 2-fold diluted control
sera were 96% (1.1%) [mean (SD)] of those in the nondiluted
sera, and in 2-fold diluted
sera from 2 patients taking diclofenac, F[T.sub.3] concentrations were 95.9% and 96.3% of those in the nondiluted
sera (Fig.
Sera were used for the quantification of [beta]-LG by ELISA.
A blinded panel of 50 samples comprising
sera from healthy persons, patients infected with other human coronaviruses (OC43 and 229E), patients infected with influenza virus, and persons who were convalescent from SARS was provided by the Health Protection Agency (HPA), United Kingdom, for the validation of our pseudotype neutralization assay.
It has been suggested (17) that quantitative assessment of Tg in TgAb-positive
sera may be achieved by determination of the recovery of known amounts of Tg added to TgAb-positive samples.
We also found that the addition of
sera protected the cells from the adverse effects of chlorpyrifos and that the effect could be reproduced by addition of albumin.
Patient 1,46 years old, and patient 2, 25 years old, had a flulike syndrome with maculopapular exanthema; WNV seroconversion was seen on a pair of
sera collected on days 3 and 16 for patient 1, and days 3 and 12 for patient 2, after onset of fever.
In
sera from healthy individuals, TfR-Tf eluted as a single peak at ~330 kDa (Fig.
Validity of interpretation criteria for standardized Western blots (immunoblots) for serodiagnosis of Lyme borreliosis based on
sera collected throughout Europe.
Briefly,
sera were treated with 1,1,2-trichloro-trifluoroethane to remove lipids, passed over a HiTrap Protein G column (Pharmacia Biotech) to remove immunoglobulins, and then passed over a HiTrap Blue column (Pharmacia Biotech) to remove human serum albumin.
Puumala and Dobrava viruses cause hemorrhagic fever with renal syndrome in Bosnia-Herzegovina: evidence of highly cross-neutralizing antibody responses in early patient
sera. J Med Virol.
Detection of cytomegalovirus DNA in
sera of liver transplant recipients.