Absolutely not; no way. A: "I mean, would you betray your co-workers for a bit of extra money?" B: "No sir! I have principles." No sir, I will not be fooled again!
Also, no sirree. Certainly not. This emphatic denial is used without regard to the gender of the person addressed. For example, No sir, I'm not taking her up on that, or Live here? No sirree. [Mid-1800s]
ˌno sirˈree!(spoken, especially American English) certainly not: We will never allow that to happen! No sir!
See also: no