Absolutely not; no way. "Siree" is an informal version of "sir" (sometimes spelled "sirree"). Primarily heard in US. A: "I mean, would you betray your coworkers for a bit of extra money?" B: "No siree! I have principles." No sirree, I will not be fooled again!
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No siree(, Bob)!
Inf. Absolutely no! (Not necessarily said to a male, and rarely to any Bob.) Bill: Do you want to sell this old rocking chair? Jane: No siree, Bob! Bill: You don't want sweet potatoes, do you? Fred: No siree!
See also: no
McGraw-Hill Dictionary of American Idioms and Phrasal Verbs. © 2002 by The McGraw-Hill Companies, Inc.