Absolutely not; no way. "Sirree" is an informal version of "sir." A: "I mean, would you betray your coworkers for a bit of extra money?" B: "No sirree! I have principles." No sirree, I will not be fooled again!
No siree(, Bob)!
Inf. Absolutely no! (Not necessarily said to a male, and rarely to any Bob.) Bill: Do you want to sell this old rocking chair? Jane: No siree, Bob! Bill: You don't want sweet potatoes, do you? Fred: No siree!
See also: no