In the HEPES 2 group, we used dextran instead of plasma to rule out the effect of extracellular HC[O.
with HEPES in the HEPES 1 group abolished PAP during the acute and sustained phases of HPV.
4] in HEPES
buffer (protein-free medium) was bound to the materials examined, probably to a pool of unspecific low-affinity binding sites on the membranes and the materials.
5 and 50 [micro]mol/L adenosine, the samples were diluted in 20 mmol/L Tris-40 mmol/L HEPES, pH 7.
Table 2 shows the results for analysis of dilutions of a urine sample with an adenosine concentration of 311 nmol/L, as determined by HPLC and ABPA and then diluted in 20 mmol/L Tris-40 mmol/L HEPES, pH 7.
0 nmol/L adenosine was diluted in 20 mmol/L Tris-40 mmol/L HEPES, pH 7.
The effects of pH on the calcium determination were examined in 100 mmol/L HEPES
buffer at various pH values (6.
Citrated plasma samples were diluted 20-fold in 20 mmol/L HEPES, pH 7.
To 20 [micro]L of a plasma sample diluted 1:20 in 20 mmol/L HEPES, pH 7.
To determine whether dopamine and dobutamine could be oxidized under these conditions, the absorbance spectra of dopamine and dobutamine were determined in the presence and absence of peroxide in a HEPES buffer, pH 6.
A much different result was seen when a twofold molar excess of 4-aminophenazone (to dopamine) was incubated with dopamine and 500 U/L peroxidase in HEPES buffer, pH 6.
1, 1, and 2 pmol digoxin equivalents) or same amount of digoxin or ouabain were dried, reconstituted with 50 [micro]L of the same HEPES
buffer, and incubated at 37 [degrees]C with 50 [micro]L of erythrocytes and 148 kBq (4 [micro]Ci)/mL [sup.
RBC is offering cGMP HEPES
, MOPS, and MES in convenient prepackaged development quantities on their new Catalog site.
After chemical treatments, cells were washed with ice-cold PBS/EDTA, and then 1 mL of ice-cold lysis buffer (50 mM HEPES
, pH 7.
Studies with proton-selective electrodes were conducted in a saline containing 2 mM HEPES
in place of the normally applied 10 mM HEPES