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Absolutely not; no way. "Sirree" is an informal version of "sir." A: "I mean, would you betray your coworkers for a bit of extra money?" B: "No sirree! I have principles." No sirree, I will not be fooled again!
No siree(, Bob)!
Inf. Absolutely no! (Not necessarily said to a male, and rarely to any Bob.) Bill: Do you want to sell this old rocking chair? Jane: No siree, Bob! Bill: You don't want sweet potatoes, do you? Fred: No siree!
See also: no
Yes siree(, Bob)!
Inf. Absolutely!; Without a doubt! (Not necessarily said to a male and not necessarily to Bob.) Mary: Do you want some more cake? Tom: Yes siree, Bob! "That was a fine turkey dinner. Yes siree!" said Uncle Henry.
See also: yes