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1. To commit suicide. Once John realized he had begun to have thoughts of killing himself, he decided to seek help.
2. To bring about one's death by performing a particular action. This phrase can be said when one's life is or could be in danger, but it is often used figuratively. I can hardly breathe—I'm going to kill myself if I go back on the treadmill right now! If they try driving home in this snow, they'll kill themselves—tell them to get a hotel room for the night.
3. To overexert oneself. Don't kill yourself trying to get the report done tonight—it's not due until next week anyway.
kill (oneself) (doing something)
To overexert oneself or overextend one's effort while doing something. Don't kill yourself trying to get the report done tonight—it's not due until next week anyway. They're killing themselves finishing the last touches on the exterior of the house before the storm hits us.
kill (oneself) laughing
To laugh hysterically and uncontrollably. The comedian had the whole audience killing themselves laughing. My ribs actually hurt from it! I was surprised by how funny my date was last night. I was killing myself laughing at some of her stories!