After incubation, the cells were transfected with 1:3 ratio of cDNA encoding FRET
probe (Turquoise--catalytically inactive EPAC--Venus): TransIT--X2 (transfection reagent from Mirusbio).
TaqMan chemistries take advantage of this and FRET
together through the addition of short single-stranded synthetic DNA probes, labeled at one end with a reporter and at the other end with its paired quencher, to a PCR reaction.
It is difficult to predict how long sea fret
will last, it depends on the air temperatures and the wind.
Les experts ont ete charges d'identifier les failles dans le controle du fret
et des colis postaux dans les aeroports.
I also fret
that this attitude to education and spelling infers that all young people are equally dim, which is simply not the case.
requires the use of two fluorescent dyes, one termed a donor and the other an acceptor.
But those with an insatiable sweet tooth shouldn't fret
about everything you haven't done yet to get ready for the holidays.
The revisionists of adolescence fret
over golf prodigy Michelle Wie, 14.
security experts, meanwhile, fret
that there will never be enough budget or manpower to sift through the rising mound of collected data.
So when I write, I don't fret
too much about whether I'm writing science fiction or fantasy.
Other program options allow for displaying chord names/guitar fret
diagrams, tablature, scaling to different sizes and automatically reformatting, rearranging/deleting verses and playing with band accompaniment.
I go to bed and fret
all night I say my prayers turn out the light I wonder how I'mgoing to pay.
5-inch color LCD panel, and there's no need to fret
about recording over images you've laid down previously because the M30K automatically finds space for you and starts recording there.
They worry and fret
about their sinfulness, as if their power to sin is greater than God's power to forgive.