4] in HEPES
buffer (protein-free medium) was bound to the materials examined, probably to a pool of unspecific low-affinity binding sites on the membranes and the materials.
3], 20 mM HEPES
, 50 [micro]g/L gentamycin, 1 uM insulin, 10 [micro]M hydrocortisone, 2% Ultroser SF and 2 mg/L of the protease inhibitor aprotinin (Fluka, Buchs, Switzerland).
3]H]adenosine was performed in a final assay volume of 300 [micro]L of 20 mmol/L Tris-40 mmol/L HEPES, pH 7.
5 and 50 [micro]mol/L adenosine, the samples were diluted in 20 mmol/L Tris-40 mmol/L HEPES, pH 7.
Table 2 shows the results for analysis of dilutions of a urine sample with an adenosine concentration of 311 nmol/L, as determined by HPLC and ABPA and then diluted in 20 mmol/L Tris-40 mmol/L HEPES, pH 7.
0 g/L bovine albumin (calcium free) in 100 mmol/L HEPES buffer (pH 7.
The effects of pH on the calcium determination were examined in 100 mmol/L HEPES buffer at various pH values (6.
To 5 [micro]L of purified proCPU (from different batches with a corresponding CPU activity of 900-2650 U/L), 10 [micro]L of thrombin-thrombomodulin in 20 mmol/L HEPES, 5 mmol/L [CaCl.
Citrated plasma samples were diluted 20-fold in 20 mmol/L HEPES, pH 7.
To 20 [micro]L of a plasma sample diluted 1:20 in 20 mmol/L HEPES, pH 7.
7), HEPES, and 4-aminoantipyrine (4-aminophenazone) were purchased from Sigma Chemical Co.
265 mmol/L) dobutamine was added to HEPES buffer in the presence of various concentrations of horseradish peroxidase.
Erythrocytes were washed and suspended to 50% cells in HEPES buffer pH 7.
1, 1, and 2 pmol digoxin equivalents) or same amount of digoxin or ouabain were dried, reconstituted with 50 [micro]L of the same HEPES buffer, and incubated at 37 [degrees]C with 50 [micro]L of erythrocytes and 148 kBq (4 [micro]Ci)/mL [sup.
The medium was applied to a HiTrap SP Sepharose HP cation-exchange column (bed volume, 5 mL; Amersham Biosciences) equilibrated with 10 mmol/L HEPES
buffer (pH 7.